If you are using Excel 97 (and later versions?) you will need to install the Analysis Tools Add-ins to make this work. Go to Tools -> Add-ins and make sure the Analysis Toolpak is check-marked. Once you do that you can use the NETWORKDAYS worksheet function to do what you need. From the Excel Help Section:
Syntax:
NETWORKDAYS(start_date,end_date,holidays)
Start_date is a date that represents the start date.
End_date is a date that represents the end date.
Holidays is an optional range of one or more dates to exclude from the working calendar, such as state and federal holidays and floating holidays.
Remark
If any argument is not a valid date, NETWORKDAYS returns the #NUM! error value.
Example
NETWORKDAYS(DATEVALUE("10/01/91"), DATEVALUE("12/01/91"), DATEVALUE("11/28/91")) equals 43.
HTH